DNA Vectors
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Filtered Search Results
Promega Nano-Glo HiBiT Extracellular Detection System, 100ml
Nano-Glo HiBiT Extracellular Detection System quantitates cell surface or secreted protein expression using a simple add-mix-read assay format and the nonlytic Nano-Glo HiBiT Extracellular Detection Reagent.
Promega DDR1-NanoLuc Fusion Vector, 20microg
The NanoLuc Kinase Fusion Vectors contain the coding region of the indicated human kinase fused to either the C- or the N-terminus of NanoLuc luciferase. Designed for use with the NanoBRET Target Engagement (TE) Intracellular Kinase Assay.
Promega TNT™ T7 Insect Cell Extract Protein Expression System Companion Vectors
Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5' and 3' ends of the gene coding region to enhance translation efficiency.
| Promoter | T7 |
|---|---|
| Restriction Site | SgfI and PmeI |
| Content And Storage | -30°C to -10°C |
| Storage Buffer | 10mM Tris HCl, 1mM EDTA (pH 8) |
| Quantity | 20 μg |
| Concentration | 100 ng/μL |
| For Use With (Application) | Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications |
Invitrogen™ BLOCK-iT™ Inducible Pol II miR RNAi Expression Vector Kit with EmGFP
The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits combine the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Promega pFC37K HiBiT CMV-neo Flexi Vector, 20microg
The pFC37K HiBiT, pFN38K HiBiT and pFN39K secHiBiT CMV-neo Flexi Vectors are Flexi Cloning vectors that append the HiBiT tag to the N or C terminus of ORFs; the N terminus for transmembrane or secreted proteins of interest.
Promega pTNT™ Vector
The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.
Invitrogen™ pPIC6α A, B, & C Pichia Vectors
pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection, high-level expression, and rapid protein purification and detection.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Promega Corporation HaloTag-Ubiquitin Fusion Vector
Fusion Vectors for the NanoBRET™ Ubiquitin Proteasome System Interaction Assays measure induced interaction between target protein and E3 ligase components VHL or CRBN with degrader compound treatment. Analyze target protein ubiquitination and recruitment to the proteasome.
Promega psiCHECK™ Vectors
psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference assays.
Promega pBiT3.1-secN[CMV/HiBiT/Blast] Vector, 20microg
The pBiT3.1-N, pBiT3.1-C and pBiT3.1-secN [CMV/HiBiT/Blast] Vectors are traditional cloning vectors for inserting proteins of interest. Cloned ORFs contain the HiBiT tag at the N or C terminus; the N terminus for transmembrane or secreted proteins.
Promega pGL4 Minimal Promoter Driven Luciferase Vectors
Firefly luciferase reporter vectors with response elements.
| Promoter | SV40 |
|---|---|
| Content And Storage | -30°C to -10°C |
| Storage Buffer | 10mM Tris HCl (pH 7.4), 1mM EDTA |
| Primer | RV Primer 3 and RV Primer 4 |
| Protein Degradation Sequence | hPEST |
| Quantity | 20 μg |
| Concentration | 1 μg/μL |
| For Use With (Application) | Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis, transient transfection experiments |
| Reporter Gene | luc2P |
| Marker Gene | Hygro |
Promega BTK-NanoLuc Fusion Vector, 20microg
The NanoLuc Kinase Fusion Vectors contain the coding region of the indicated human kinase fused to either the C- or the N-terminus of NanoLuc luciferase. Designed for use with the NanoBRET Target Engagement (TE) Intracellular Kinase Assay.
Promega pCI and pCI-neo Mammalian Expression Vectors
Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.
| Promoter | CMV |
|---|---|
| Content And Storage | -30°C to -10°C |
| Storage Buffer | TE Buffer (pH 8) |
| Quantity | 20 μg |
| For Use With (Application) | Promote constitutive expression of cloned DNA inserts in Mammalian cells, can also be used to synthesize transcripts in-vitro using the T7 RNA Polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication |
Invitrogen™ ViraPower™ BacMam Expression System
The ViraPower™ BacMam Expression System provides BacMam pCMV-Dest Vector, Cellfectin™ II Reagent, and MAX Efficiency™ DH10Bac™ Competent Cells for easy recombination-based cloning and baculovirus-based expression of a target gene.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | -30°C to -10°C |
|---|---|
| For Use With (Application) | Cell imaging of Protein localization or trafficking in conjunction with the fluorescent HaloTag™ ligands by using expression of HaloTag™ fusion Proteins in E. coli and cell-free Protein expression |